Boss Sam Allardyce has made his first selection as national team boss and opted for a trio of City players for the Group F game in Trnava.
Both Sterling and Stones were again impressive as City defeated West Ham 3-1 at the Etihad Stadium to go top of the Premier League.
Full England squad:
Goalkeepers: Fraser Forster (Southampton), Joe Hart (Manchester City), Tom Heaton (Burnley)
Defenders: Gary Cahill (Chelsea), Nathaniel Clyne (Liverpool), Phil Jagielka (Everton), Danny Rose (Tottenham Hotspur), Luke Shaw (Manchester United), Chris Smalling (Manchester United), John Stones (Manchester City), Kyle Walker (Tottenham Hotspur)
Midfielders: Dele Alli (Tottenham Hotspur), Michail Antonio (West Ham United), Eric Dier (Tottenham Hotspur), Danny Drinkwater (Leicester City), Jordan Henderson (Liverpool), Adam Lallana (Liverpool), Wayne Rooney (Manchester United), Raheem Sterling (Manchester City), Theo Walcott (Arsenal)
Strikers: Harry Kane (Tottenham Hotspur), Daniel Sturridge (Liverpool), Jamie Vardy (Leicester City)